Invitrogen™ Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA514520

Catalog No. PIMA514520

Description

Staining of formalin-fixed tissues requires boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes. Recommended positive controls: IHC (P) - Human tonsil and testis tissue, common marmoset spleen tissue, rat esophagus, small intestine and liver tissue, mouse embryonic skin tissue IHC (F) - Rat lymph node tissue, transgenic mouse spinal cord tissue ICC/IF - HAP1 cells, human cardiac stem cells, HEp-2 cells, rat cardiomyocytes Flow - HAP1 cells.

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

Specifications

• Antigen: Ki-67
• Classification: Recombinant Monoclonal
• Concentration: 0.031 mg/mL
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.2
• Gene Accession No.: E9PVX6, P46013
• Gene Symbols: Mki67
• Immunogen: Synthetic peptide within Human Ki67 aa 1200-1300.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: SP6
• Conjugate: Unconjugated
• Gene: Mki67
• Gene Alias: antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 17345, 291234, 4288
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid