Invitrogen™ Ki-67 Recombinant Rat Monoclonal Antibody (SolA15), Invitrogen™

Catalog No.	Quantity
74-000-8T20UG	20 μL
74-000-8T	100 μL

Catalog No. 74-000-8T20UG Supplier Invitrogen™ Supplier No. 740008T20UG

Description

Rat Recombinant Monoclonal Antibody

Recombinant Rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

Specifications

• Antigen: Ki-67
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: SolA15
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene: Mki67
• Gene Accession No.: E9PVX6, P46013
• Gene Alias: antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
• Gene Symbols: Mki67
• Host Species: Rat
• Purification Method: Affinity Chromatography
• Quantity: 20 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17345, 4288
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ