Ki67 Rat anti-Human, Clone: OTI3B6, lyophilized, TrueMAB™

Rat Monoclonal Antibody

Supplier:  OriGene CF801343

Catalog No. 50-166-5558

Description

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product No. 7KMWCO

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

Specifications

• Antigen: Ki67
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: MKI67
• Gene Alias: KIA, MIB-, MIB-1, PPP1R105
• Host Species: Rat
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4288
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin)
• Clone: OTI3B6
• Formulation: PBS with 8% trehalose and no preservative; pH 7.3
• Gene Accession No.: P46013
• Gene Symbols: MKI67
• Immunogen: Human recombit protein fragment corresponding to amino acids 1160-1493 of human MKI67 produced in E.coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1