Invitrogen™ Kinesin 5B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1643

Catalog No. PA1643

Description

PA1-643 detects kinesin 5B from mouse and human samples. This antibody is specific for kinesin 5B and does not detect other kinesin isotypes. PA1-643 has been successfully used in Western blot and immunofluorescent procedures. By Western blot, this antibody detects an ~110 kDa protein representing kinesin 5B protein. The PA1-643 immunizing peptide corresponds to amino acid residues 376-396 from human kinesin 5B. This sequence is 95% and 90% conserved for mouse and rat, respectively. This peptide (Cat. # PEP-198) is available for use in neutralization and control experiments.

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.

Specifications

• Antigen: Kinesin 5B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: KIF5B
• Gene Alias: AL022807; Conventional kinesin heavy chain; EMBL:ABC25059.1}; epididymis secretory protein Li 61; HEL-S-61; Khc; Khcs; KIF5A; KIF5B; kif5b {ECO:0000312; kinesin 1 (110-120kD); kinesin family member 5A; kinesin family member 5B; kinesin heavy chain; kinesin heavy chain member 5B, ubiquitous; kinesin-1 heavy chain; KINH; KNS; Kns1; ubiquitous kinesin heavy chain; UKHC; U-KHC; Unknown (protein for IMAGE:7944745)
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 117550, 16573, 3799
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1 mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P33176, Q2PQA9, Q61768
• Gene Symbols: KIF5B
• Immunogen: Synthetic Peptide: C I(376) D E Q F D K E K A N L E A F T V D K D I(396).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG