Invitrogen™ Kir2.1 (KCNJ2) Monoclonal Antibody (S112), PE

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545418

Catalog No. PIMA545418

Description

1 μg/mL of MA5-45418 was sufficient for detection of Kir2.1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 45kDa. No cross-reactivity against Kir2.2 or Kir2.3.

The KIR2 subunit family includes 2. 1, 2. 2, 2. 3 and 2. 4. Unlike G protein-coupled KIR3 subunits, KIR2. 1 requires both phosphorylation by PKA and ATP hydrolysis for functional activity. KIR2. 1 is expressed in the superior and inferior collicula and the pontine region of the brain, where it moderates synaptic transmission, like many other potassium channels. In the placenta, KIR2. 1 is expressed throughout gestation in cytotrophoblast cells. In the kidney, KIR2. 1 colocalizes with KIR5. 1 in the proximal tubule. KIR2. 1, 2. 2 and 2. 3 associate with the membrane-associated guanylate kinase synapseassociated protein 97 in the cerebellum and heart. Phosphorylation of KIR2. 2 by protein kinase A inhibits the associates with SAP97.

Specifications

• Antigen: Kir2.1 (KCNJ2)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: P35561, P63252, Q64273
• Gene Symbols: Kcnj2
• Immunogen: Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry, Microarray
• Clone: S112
• Conjugate: PE
• Gene: Kcnj2
• Gene Alias: ATFB9; cardiac inward rectifier KIR2.1; cardiac inward rectifier potassium channel; EGK_08925; HHBIRK1; HHIRK 1; HHIRK1; HIRK 1; hIRK1; Inward rectifier K; inward rectifier K(+) channel Kir2.1; inward rectifier K+ channel KIR2.1; inward rectifier potassium channel; inward rectifier potassium channel 2; inwardly rectifying potassium channel Kir2.1; IRK 1; IRK1; IRK-1; IRK2; Kcnf1; KCNJ2; KIR2.1; LQT 7; LQT7; Potassium channel, inwardly rectifying subfamily J member 2; potassium channel, inwardly rectifying subfamily J, member 2; potassium inwardly rectifying channel subfamily J member 2; potassium inwardly-rectifying channel, subfamily J, member 2; potassium voltage-gated channel subfamily J member 2; RBL-IRK1; SQT 3; SQT3
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 16518, 29712, 3759
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov