Invitrogen™ KIR2D Monoclonal Antibody (NKVFS1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA516887

Catalog No. PIMA516887

Description

Clone NKVFS1 recognizes the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4). This clone is reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several framework genes are found in all haplotypes. The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response.

Specifications

• Antigen: KIR2D
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: P43626, P43631, P43632, Q14953, Q14954, Q99706
• Gene Symbols: KIR2DL1, KIR2DL4, KIR2DS1, KIR2DS2, KIR2DS4, KIR2DS5
• Immunogen: Proprietary Immunogen.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunoprecipitation, Western Blot
• Clone: NKVFS1
• Conjugate: Unconjugated
• Gene: KIR2DS4
• Gene Alias: 183ActI; CD158; CD158 antigen-like family member A; CD158 antigen-like family member G; CD158 antigen-like family member H; CD158 antigen-like family member I; CD158 antigen-like family member J; CD158A; CD158b; CD158B1; CD158G; CD158H; CD158I; CD158j; cl-49; killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 1; killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 1; killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 2; killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 4; killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 5; killer cell immunoglobulin-like receptor 2DL1; Killer cell immunoglobulin-like receptor 2DS1; killer cell immunoglobulin-like receptor 2DS2; Killer cell immunoglobulin-like receptor 2DS4; killer cell immunoglobulin-like receptor 2DS5; killer cell immunoglobulin-like receptor KIR2DS2; killer cell immunoglobulin-like receptor KIRDS1; killer cell immunoglobulin-like receptor two domains short cytoplasmic tail 1; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 1; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 2; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5; killer Ig receptor; killer inhibitory receptor 2-2-1; killer inhibitory receptor 4-1-2; killer-cell Ig-like receptor; killer-cell immunoglobulin-like receptor; killer-cell immunoglobulin-like receptor two domains short tail 2 protein; KIR antigen 2DS4; KIR1D; KIR221; KIR2DL1; KIR2DP1DL1; KIR2DS1; KIR2DS2; KIR-2DS2; KIR2DS4; KIR-2DS4; KIR2DS5; KIR412; KIR-K64; KKA3; MHC class I NK cell receptor; MHC class I NK cell receptor Eb6 ActI; natural killer associated transcript 5; natural killer cell inhibitory receptor; natural killer cell receptor; natural killer-associated transcript 1; Natural killer-associated transcript 5; Natural killer-associated transcript 8; natural killer-associated transcript 9; NK receptor 183 ActI; NKAT; NKAT1; NKAT-1; NKAT5; NKAT-5; NKAT6; NKAT8; NKAT-8; NKAT9; NKAT-9; p50 killer cell activating receptor KAR-K1d; p50.1; p50.3; p58 killer cell inhibitory receptor KIR-K64; p58 killer cell inhibitory receptor KIR-K7a; p58 natural killer cell receptor clone CL-49; P58 natural killer cell receptor clones CL-39/CL-17; p58 natural killer cell receptor clones CL-42/47.11; p58 NK cell inhibitory receptor NKR-K6; p58 NK receptor CL-39/CL-17; p58 NK receptor CL-42/47.11; p58 NK receptor CL-49; p58.1; p58.1 MHC class-I-specific NK receptor; XXbac-BCX195L8.1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100132285, 3802, 3805, 3806, 3809, 3810
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid