Invitrogen™ Kir3.4 (KCNJ5) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5119662

Catalog No. PIPA5119662

Description

Positive Control: Mouse heart tissue, rat heart tissue, 293T, HepG2, LOVO, human lung cancer tissue, human pancreas tissue, mouse pancreas tissue. Subcellular Location: Membrane.

G protein-coupled inwardly rectifying potassium channels (Kir3.1 through Kir3.4) are coupled to numerous neurotransmitter receptors in the brain and are abundantly expressed in the olfactory bulb, hippocampus, neocortex, dentate gyrus, cerebellar cortex and thalamus regions of the brain. Also known as GIRK, Kir3 potassium channels localize to the soma and dendrites as well as axons of neurons. Liberated Gbg subunits from G protein heterotrimers bind to and regulate Kir3 channel activity. Gb3- and Gb4-containing Gbg dimers bind directly to cytoplasmic domains of Kir3 proteins and increase the K+ current while Gb5-containing Gbg dimers inhibit Kir3 K+ current. Kir3 activity is also inhibited by tyrosine phosphorylation. Brain-derived neurotrophic factor activates receptor tyrosine kinase B, which then phosphorylates Kir3 tyrosine residues, effectively inactivating the Kir3 channels.

Specifications

• Antigen: Kir3.4 (KCNJ5)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: KCNJ5
• Gene Alias: cardiac ATP-sensitive potassium channel; cardiac inward rectifier; CIR; G protein-activated inward rectifier potassium channel 4; GIRK4; GIRK-4; Heart KATP channel; inward rectifier K(+) channel Kir3.4; inward rectifier K+ channel KIR3.4; IRK4; IRK-4; KATP1; KATP-1; KCNJ5; Kir3.4; LQT13; Potassium channel, inwardly rectifying subfamily J member 5; potassium channel, inwardly rectifying subfamily J, member 5; potassium inwardly-rectifying channel, subfamily J, member 5; potassium voltage-gated channel subfamily J member 5
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16521, 29713, 3762
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.2% BSA, 50% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P48544, P48545, P48548
• Gene Symbols: KCNJ5
• Immunogen: Synthetic peptide within N-terminal human Kir3.4.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov