Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Modifying Enzymes
  5. Promega Klenow Fragment, DNA Polymerase I

Promega Klenow Fragment, DNA Polymerase I

Catalog No. PRM2201 Shop All Promega Corporation Products
Change view
Click to view available options
Quantity:
150 U
Concentration:
5 U/μL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity Concentration
PR-M2201 150 U 5 U/μL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Catalog No. PR-M2201 Supplier Promega Supplier No. M2201
Only null left
Add to Cart
Add to Cart
This item is not returnable. View return policy

Description

A DNA-dependent DNA polymerase that lacks the 5'→3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'→3' polymerase, 3'→5' exonuclease and strand displacement activities.

  • DNA-Dependent DNA Polymerase that Lacks the 5′→3′ Exonuclease Activity
  • Use in a variety of molecular applications
  • Heat-inactivate by heating at 75°C for 10 minutes
  • Active in many Promega 1X restriction enzyme buffers
  • Supplied at a concentration of 5u/μL

†DNA polymerase kit that includes 5μmoles each of dATP, dCTP, dGTP, and dTTP at 10mM in water.

Specifications

Concentration 5 U/μL
Color Colorless
Components 10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
Purity >80%
pH 7.2
For Use With (Application) Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 150 U
Product Type DNA Polymerase I Large (Klenow) Fragment
Molecular Weight (g/mol) 68
Form Liquid
Show More Show Less
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.