KLF4, Rabbit anti-Mouse, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
ABE286025UL	MilliporeSigma ABE286025UL	25 μL
ABE2860100	MilliporeSigma ABE2860100UL	100 μL

Description

Anti-KLF4, Cat. No. ABE2860, is a highly specific rabbit polyclonal antibody that targets Krueppel-like factor 4 (Klf4) and has been tested for use in ChIP-seq, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

Krueppel-like factor 4 (UniProt: Q60793; also known as Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4) is encoded by the Klf4 (also known as Ezf, Gklf, Zie) gene (Gene ID: 16600) in murine species. Klf4 is a member of the krueppel C2H2-type zinc-finger protein family that regulates multiple biological processes, including proliferation, differentiation, development, and apoptosis. KLF4 is a developmentally regulated transcription factor that plays an important role in maintaining the pluripotent state in embryonic stem cells (ESCs) and can be used as a marker of the pluripotent state for cells in culture. It can serve both as activator and as repressor depending upon its environment, but its most prominent function is growth suppression. Interestingly, increased expression of Klf4 leads to an increase in expression of P21WAF1/CIP1, which is an important checkpoint protein that inhibits cell cycle progression and is essential in mediating the cell cycle arrest at both the G1/S and G2/M boundaries. Klf4 also mediates the normal functions of the tumor suppressor p53 and contributes to the down-regulation of p53/TP53 transcription. Mutations in Klf4 gene can lead to unchecked growth and tumor formation. KLF4 is shown to be often mutated in many colon cancer cell lines and the lack of Klf4 expression is correlated with colorectal cancer pathogenesis. Elevated expression of Klf4 gene has been linked to gastric cancer, breast cancer, and squamous cell carcinomas. KLF4 is reported to target the primary miR1-2 loop promoter and stimulates miR-1 expression. Loss of KLF4 expression is considered as a mechanistic link between aggressive prostate cancer progression and low canonical AR output through miR-1 inactivation. (Ref.: Jiang, J., et al. (2008). Nat. Cell Biol. 10(3); 353-360; Siu, MK et al. (2016). Oncogenesis 5: e282).

Specifications

• Antigen: KLF4
• Applications: ChIP Assay, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Research Discipline: Epigenetics & Nuclear Function
• Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
• Gene ID (Entrez): NP_034767
• Immunogen: GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Molecular Weight of Antigen: ∽55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

• Dilution: Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
• Classification: Polyclonal
• Form: Serum
• Regulatory Status: RUO
• Target Species: Mouse
• Gene Alias: Krueppel-like factor 4;Epithelial zinc finger protein EZF;Gut-enriched krueppel-like factor
• Gene Symbols: Klf4;Ezf;Gklf;Zie
• Isotype: IgG
• Purification Method: Unpurified
• Test Specificity: This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.