Collagen II Ab-3 Mouse Monoclonal Antibody, Epredia™

Obtain accurate, reproducible results in immunohistochemistry and immunofluorescence experiments with Epredia™ Collagen II Ab-3, Mouse Monoclonal Antibody.

Supplier:  Epredia MS306P1

Catalog No. MS306P1

Description

Host Species: Mouse

Clone: 6B3

Isotype: IgG1

Species Reactivity: Human, Mouse, Chicken and Salamander. Others not known.

Epitope: Triple helix of type II collagen

Immunogen: Purified type II collagen; obtained from the adult chicken sternum by limited pepsinization

Positive Control: Cartilage in lung or fetus

Cellular Localization: Cartilage matrix

In Western blotting, Ab-3 reacts with the TC^A fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. Immunoblotting of CNBr peptides of collagen II shows that MAb 6B3 reacts with CB 11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. In Western blotting (but not ELISA), Ab-3 recognizes alpha1(II) as well as alpha3(XI) chain both of which have identical primary structure.

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Western Blotting (Not verified)
• Immunofluorescence

Note:

USA: RUO; Int'l: RUO

Specifications

• Antigen: Collagen II Ab-3
• Classification: Monoclonal
• Concentration: 200μg/mL
• Host Species: Mouse
• Quantity: 500 μL
• Research Discipline: Extracellular Matrix
• Target Species: Chicken, Human, Mouse, Salamander
• Isotype: IgG1

• Applications: Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 6B3
• Conjugate: Unconjugated
• Immunogen: Purified type II collagen; obtained from the adult chicken sternum by limited pepsinization
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Concentrated