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Collagen II Ab-3 Mouse Monoclonal Antibody, Epredia™

Catalog No. MS306PABX Shop All Epredia Products
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Quantity:
100 μL
200 μL
500 μL
1 mL
7 mL
Form:
Concentrated
Prediluted
Concentration:
1mg/mL
200μg/mL
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Catalog No. Quantity Form Concentration
MS306PABX 200 μL Concentrated 1mg/mL
MS306P1ABX 100 μL Concentrated 1mg/mL
MS306P 1 mL Concentrated 200μg/mL
MS306R7 7 mL Prediluted -
MS306P0 100 μL Concentrated 200μg/mL
MS306P1 500 μL Concentrated 200μg/mL
MS306R1 1 mL Prediluted -
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Catalog No. MS306PABX Supplier Epredia Supplier No. MS306PABX

Description

Obtain accurate, reproducible results in immunohistochemistry and immunofluorescence experiments with Epredia™ Collagen II Ab-3, Mouse Monoclonal Antibody.

Host Species: Mouse

Clone: 6B3

Isotype: IgG1

Species Reactivity: Human, Mouse, Chicken and Salamander. Others not known.

Epitope: Triple helix of type II collagen

Immunogen: Purified type II collagen; obtained from the adult chicken sternum by limited pepsinization

Positive Control: Cartilage in lung or fetus

Cellular Localization: Cartilage matrix

In Western blotting, Ab-3 reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It also reacts with pepsin-digested type II collagen. Immunoblotting of CNBr peptides of collagen II shows that MAb 6B3 reacts with CB 11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. In Western blotting (but not ELISA), Ab-3 recognizes alpha1(II) as well as alpha3(XI) chain both of which have identical primary structure.

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting (Not verified)
  • Immunofluorescence

Note:

USA: RUO; Int'l: RUO

Specifications

Antigen Collagen II Ab-3
Applications Immunofluorescence, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone 6B3
Concentration 1mg/mL
Conjugate Unconjugated
Host Species Mouse
Immunogen Purified type II collagen; obtained from the adult chicken sternum by limited pepsinization
Quantity 200 μL
Regulatory Status RUO
Research Discipline Extracellular Matrix
Primary or Secondary Primary
Target Species Chicken, Human, Mouse, Salamander
Form Concentrated
Isotype IgG1
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