Epredia™ Lab Vision™ Cyclin B1 Ab-2 Rabbit Polyclonal Antibody with BSA and azide

Ensure accurate, reproducible results in immunohistochemistry, western blotting and immunoprecipitation experiments.

Supplier:  Epredia™ RB008PABX

Catalog No. RB008PABX

Description

In mammals, cyclin B associates with inactive p34^cdc2 which facilitates phosphorylation of p34^cdc2 at aa 14^Thr and 15^Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34^cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14^Thr and 15^Tyr of p34^cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34^cdc2 plays a critical role in G2 to M transition.

Host Species: Rabbit

Isotype: IgG

Species Reactivity: Human, Mouse and Rat. Others not known.

Epitope: C-terminal

Immunogen: A synthetic peptide derived from the C-terminus of human cyclin B1

Molecular Weight: 62 kDa

Positive Control: Jurkat, Ls174T, HeLa cells; Breast CA

Cellular Localization: Cytoplasmic

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Western Blotting
• Immunoprecipitation (Native and denatured)

Note:

USA: RUO; Int'l: RUO

Specifications

• Antigen: Cyclin B1 Ab-2
• Classification: Polyclonal
• Concentration: 1mg/mL
• Host Species: Rabbit
• Quantity: 1 mL
• Research Discipline: Cell Cycle
• Target Species: Human, Mouse
• Isotype: IgG

• Applications: Immunoprecipitation
• Clone: Polyclonal
• Conjugate: Unconjugated
• Immunogen: A synthetic peptide derived from the C-terminus of human cyclin B1
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Concentrated