Learn More
Lac I Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody
Supplier: Rockland Immunochemicals 600401B04

Description
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100% homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined.
The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.Specifications
Lac I | |
Polyclonal | |
Unconjugated | |
Rabbit | |
RUO | |
Bacteria | |
Antibody |
ELISA, Western Blot | |
1 mg/mL | |
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2 | |
100 μg | |
Primary | |
-20°C, Avoid Freeze/Thaw Cycles | |
Liquid |
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.