Invitrogen™ Lambda light chain Monoclonal Antibody (1-155-2), APC, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-153-71	Invitrogen™ 17999042	100 Tests

Description

Description: The monoclonal antibody 1-155-2 recognizes the lambda light chain of human immunoglobulin. Approximately 22-kDa, the Ig lambda chain is comprised of one variable and one constant region. Two lambda chains together with the heavy chain comprise the whole Ig structure. Expression of Ig lambda is typically found in less than half of B lymphocytes, and has been associated with neoplastic lymphomas. Applications Reported: This 1-155-2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1-155-2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five types) and two light chains (kappa, lambda; both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30. Antibodies to the kappa light chain are reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Specifications

• Antigen: Lambda light chain
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01701, P0CG04
• Gene ID (Entrez): 28820, 3535, 3537, 3546
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 1-155-2
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human
• Gene: IGLC1
• Gene Alias: Bence Jones Protein; BJP; Clambda1; Ig lambda chain V-I region BL2; IgL; Igl@; IGLC; IGLC 1/2/3; IGLC1; Igl-C1; IGLL5; IGLV; IGLV@; immmunoglobulin lambda light chain variable region; Immunoglobulin L; immunoglobulin lambda chain complex; immunoglobulin lambda constant 1; immunoglobulin lambda constant 7; immunoglobulin lambda light chain; immunoglobulin lambda light chain constant region; immunoglobulin lambda light chain V-J region; immunoglobulin lambda variable cluster; Lambda LC; lambda-immunoglobulin; LOC100060608; Mcg Marker; Paraprotein
• Gene Symbols: IGL, IGLC1, IGLV, IGLV1-51
• Product Type: Antibody