MilliporeSigma™ Lamin A/C, Mouse, Unlabeled, Clone: 2E8.2,

Catalog No.	Quantity	Conjugate
MABT538MI	100 μg	Unconjugated

Catalog No. MABT538MI Supplier MilliporeSigma™ Supplier No. MABT538

Description

Mouse Monoclonal Antibody

Lamin-A is a key component of the nuclear lamina, a protein network underlying the inner membrane that determines nuclear shape and size. The same gene (Lmna, Dhe, or Lamn1 in murine species; Gene ID 16905) that encodes lamin-A also codes for the alternatively spliced prelamin-C isoforms (UniProt P48678-2 and P48678-3). Murine lamin-A (UniProt P48678-1) is initially produced as a 665-amino acid prelamin A, which is further processed posttranslationally by four enzymatic steps to yield the mature Lamin-A. Farnesylation of Cys662, endoproteolytic cleavage of the last three amino acids (a.a. 663-665), carboxyl methylation of Cys662, and the final endoproteolytic removal of the remaining propeptide sequence (a.a. 648-662), including the farnesylated Cys662, catalyzed by the ER membrane zinc metalloproteinase ZMPSTE24.

Specifications

• Antigen: Lamin A/C
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 2E8.2
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: LMNA, LMN1
• Gene Accession No.: P02545
• Host Species: Mouse
• Immunogen: GST-tagged recombinant fragment within the C-terminal half of human Lamin A.
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_733821
• Test Specificity: Clone 2E8.2 immunostained nuclear membrane in human tissue sections.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.