Invitrogen™ Laminin alpha-2 Monoclonal Antibody (CL3450)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA524656

Catalog No. PIMA524656

Description

Immunogen sequence: DKLKPIKELE DNLKKNISEI KELINQARKQ ANSIKVSVSS GGDCIRTYKP EIKKGSYNNI VVNVKTAVAD NLLFYLGSAK FIDFLAIEMR KGKVSFLWDV GSGVGRVEYP DLTIDDSYWY RIVASRTGRN GT Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 98%. Binds to an epitope located within the peptide sequence DKLKPIKELEDNLKK as determined by overlapping synthetic peptides.

Laminin is a non-collagenous protein of basement membranes. By rotary shadowing it has a cross-like shape, consisting of one long arm, (400 kD) and three short arms (200 kD). Antibodies to laminin localize exclusively in basement membranes. Laminin is found throughout the basement membrane, but the reaction with the antibodies to laminin is more intense in the lamina lucida, the part of the basement membrane adjacent to the cell membrane. Laminin binds to type IV collagen, a component exclusively localized in the basement membrane. Laminin has been shown to play a role in cell adhesion and attachment in vivo and in vitro.

Specifications

• Antigen: Laminin alpha-2
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P24043, Q60675
• Gene Symbols: Lama2
• Immunogen: Recombinant protein corresponding to Human Laminin alpha-2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: CL3450
• Conjugate: Unconjugated
• Gene: Lama2
• Gene Alias: 5830440B04; dy; dystrophia muscularis; Lama2; laminin alpha-2; laminin M; Laminin M chain; laminin subunit alpha 2; laminin subunit alpha-2; laminin, alpha 2; laminin-12 subunit alpha; Laminin-2 subunit alpha; laminin-4 subunit alpha; LAMM; mer; merosin; Merosin heavy chain; mKIAA4087
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 16773, 309368, 3908
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid