Invitrogen™ LAMP-2A Polyclonal Antibody (AMC2)

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 512200

Catalog No. PI512200

Description

This antibody was tested for specificity in rat and mouse (it does not cross-react with the human variant) and was demonstrated to be highly selective for LAMP-2A without cross-reacting with LAMP-2B or C. This protein was previously known as lgp96. In western blotting this antibody was used at 90 ng/mL with 100 μg of rat liver lysosomal protein. This antibody is also able to completely block transport of cytosolic proteins into lysosomes in rat-derived cells.

LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon's disease).

Specifications

• Antigen: LAMP-2A
• Classification: Polyclonal
• Concentration: 0.25 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene Accession No.: P17046, P17047
• Gene Symbols: LAMP2
• Immunogen: A synthetic peptide of the cytosolic tail of rat LAMP-2A (GLKRHHTGYEQF).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Inhibition Assays, Western Blot
• Clone: AMC2
• Conjugate: Unconjugated
• Gene: LAMP2
• Gene Alias: CD107 antigen-like family member B; CD107b; EGK_20887; Igp96; Lamp II; LAMP2; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; Lamp-2a; Lamp-2b; Lamp-2c; LAMPB; LGP110; LGP-110; LGP-96; LGP-B; lysosomal associated membrane protein 2; lysosomal membrane glycoprotein 2; lysosomal membrane glycoprotein type B; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; lysosome-associated membrane protein 2; Mac3; RP23-193O17.2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16784, 24944
• Content And Storage: -20°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov