Invitrogen™ LAMP5 (BAD-LAMP) Monoclonal Antibody (34.2), eBioscience™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 14977880

Catalog No. 14-977-880

Description

The monoclonal antibody 34.2 recognizes human lysosome-associated membrane protein 5 (LAMP5), a member of the LAMP family. LAMP5 differs from other LAMP family members in that its expression is more restricted and is referred to as brain and dendritic cell-associated LAMP-like molecule (BAD-LAMP). In humans, BAD-LAMP is expressed in post-natal neurons and non-activated plasmacytoid dendritic cells. Expression in neurons is localized to intracellular vesicles distributed in specific microdomains along neurites and may play a role in endocytosis. BAD-LAMP is localized to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of plasmacytoid dendritic cells and is lost upon TLR activation. This 34.2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high or low pH antigen retrieval and can be used at less than or equal to 1 μg/mL. This 34.2 antibody has been tested by western blot analysis of reduced SK-N-SH cell lysates and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Lysosome-associated membrane protein 5 (LAMP5) is a member of the LAMP family. LAMP5 differs from other LAMP family members in that its expression is more restricted and is referred to as brain and dendritic cell-associated LAMP-like molecule (BAD-LAMP). In humans, BAD-LAMP is expressed in post-natal neurons and non-activated plasmacytoid dendritic cells. Expression in neurons is localized to intracellular vesicles distributed in specific microdomains along neurites and may play a role in endocytosis. BAD-LAMP is localized to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of plasmacytoid dendritic cells and is lost upon TLR activation.

Specifications

• Antigen: LAMP5 (BAD-LAMP)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% Sodium Azide; pH 7.2
• Gene Accession No.: Q9UJQ1
• Gene Symbols: LAMP5
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 24141
• Content And Storage: 4°C
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: 34.2
• Conjugate: Unconjugated
• Gene: LAMP5
• Gene Alias: 3110035N03Rik; 6330527O06Rik; BADLAMP; BAD-LAMP; brain and dendritic cell associated LAMP; brain and dendritic cell-associated LAMP; Brain-associated LAMP-like protein; C20orf103; LAMP family protein C20orf103; LAMP family protein C20orf103 homolog; LAMP5; LAMP-5; lysosomal associated membrane protein family member 5; lysosomal-associated membrane protein family, member 5; lysosome-associated membrane glycoprotein 5; Lysosome-associated membrane protein 5; Protein C20orf103; RGD1306991; UNC-46
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ