Invitrogen™ LAMP5 (BAD-LAMP) Monoclonal Antibody (34.2), eFluor™ 660, eBioscience™

Catalog No.	Quantity
50-209-0076	25 μg

Catalog No. 50-209-0076 Supplier Invitrogen™ Supplier No. 50977880

Description

Rat Monoclonal Antibody

The monoclonal antibody 34.2 recognizes human lysosome-associated membrane protein 5 (LAMP5), a member of the LAMP family. LAMP5 differs from other LAMP family members in that its expression is more restricted and is referred to as brain and dendritic cell-associated LAMP-like molecule (BAD-LAMP). In humans, BAD-LAMP is expressed in post-natal neurons and non-activated plasmacytoid dendritic cells. Expression in neurons is localized to intracellular vesicles distributed in specific microdomains along neurites and may play a role in endocytosis. BAD-LAMP is localized to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of plasmacytoid dendritic cells and is lost upon TLR activation. This 34.2 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. This 34.2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 20 μg/mL.eFluor 660 is a replacement for Alexa Fluor 647. eFluor 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Filt...

Lysosome-associated membrane protein 5 (LAMP5) is a member of the LAMP family. LAMP5 differs from other LAMP family members in that its expression is more restricted and is referred to as brain and dendritic cell-associated LAMP-like molecule (BAD-LAMP). In humans, BAD-LAMP is expressed in post-natal neurons and non-activated plasmacytoid dendritic cells. Expression in neurons is localized to intracellular vesicles distributed in specific microdomains along neurites and may play a role in endocytosis. BAD-LAMP is localized to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of plasmacytoid dendritic cells and is lost upon TLR activation.

Specifications

• Antigen: LAMP5 (BAD-LAMP)
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Monoclonal
• Clone: 34.2
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 660
• Formulation: PBS with 0.09% Sodium Azide; pH 7.2
• Gene: LAMP5
• Gene Accession No.: Q9UJQ1
• Gene Alias: 3110035N03Rik; 6330527O06Rik; BADLAMP; BAD-LAMP; brain and dendritic cell associated LAMP; brain and dendritic cell-associated LAMP; Brain-associated LAMP-like protein; C20orf103; LAMP family protein C20orf103; LAMP family protein C20orf103 homolog; LAMP5; LAMP-5; lysosomal associated membrane protein family member 5; lysosomal-associated membrane protein family, member 5; lysosome-associated membrane glycoprotein 5; Lysosome-associated membrane protein 5; Protein C20orf103; RGD1306991; UNC-46
• Gene Symbols: LAMP5
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 24141
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

Frequently Asked Questions (FAQs)

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

Is the eFluor 660 fluorochrome compatible with Anti-Cy5/Alexa Fluor 647 beads?

Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.