Invitrogen™ LAMP5 (BAD-LAMP) Monoclonal Antibody (34.2), eFluor™ 660, eBioscience™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 50977880

Catalog No. 50-209-0076

Description

The monoclonal antibody 34.2 recognizes human lysosome-associated membrane protein 5 (LAMP5), a member of the LAMP family. LAMP5 differs from other LAMP family members in that its expression is more restricted and is referred to as brain and dendritic cell-associated LAMP-like molecule (BAD-LAMP). In humans, BAD-LAMP is expressed in post-natal neurons and non-activated plasmacytoid dendritic cells. Expression in neurons is localized to intracellular vesicles distributed in specific microdomains along neurites and may play a role in endocytosis. BAD-LAMP is localized to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of plasmacytoid dendritic cells and is lost upon TLR activation. This 34.2 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. This 34.2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 20 μg/mL.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Filt...

Lysosome-associated membrane protein 5 (LAMP5) is a member of the LAMP family. LAMP5 differs from other LAMP family members in that its expression is more restricted and is referred to as brain and dendritic cell-associated LAMP-like molecule (BAD-LAMP). In humans, BAD-LAMP is expressed in post-natal neurons and non-activated plasmacytoid dendritic cells. Expression in neurons is localized to intracellular vesicles distributed in specific microdomains along neurites and may play a role in endocytosis. BAD-LAMP is localized to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) of plasmacytoid dendritic cells and is lost upon TLR activation.

Specifications

• Antigen: LAMP5 (BAD-LAMP)
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% Sodium Azide; pH 7.2
• Gene Accession No.: Q9UJQ1
• Gene Symbols: LAMP5
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 24141
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
• Clone: 34.2
• Conjugate: eFluor 660
• Gene: LAMP5
• Gene Alias: 3110035N03Rik; 6330527O06Rik; BADLAMP; BAD-LAMP; brain and dendritic cell associated LAMP; brain and dendritic cell-associated LAMP; Brain-associated LAMP-like protein; C20orf103; LAMP family protein C20orf103; LAMP family protein C20orf103 homolog; LAMP5; LAMP-5; lysosomal associated membrane protein family member 5; lysosomal-associated membrane protein family, member 5; lysosome-associated membrane glycoprotein 5; Lysosome-associated membrane protein 5; Protein C20orf103; RGD1306991; UNC-46
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ