LAP (TGF-beta 1) Rat anti-Mouse, APC, Clone: 860206, R&D Systems™
Rat Monoclonal Antibody
Manufacturer: R&D Systems FAB8118A
DescriptionTGF-β1 (Transforming Growth Factor beta 1) is one of three closely related mammalian members of the large TGF-β superfamily that share a characteristic cystine knot structure (1-7). TGF-β1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-β isoform has some non-redundant functions; for TGF-β1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF-β1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 249 aa Latency-Associated Peptide (LAP) and a C-terminal 112 aa mature TGF-β1 (8, 9). Disulfide-linked homodimers of LAP and TGF-β1 remain non-covalently associated after secretion, forming the small latent TGF-β1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-β binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-β is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-β1 shares 100% aa identity with pig, dog and cow TGF-β1, and 99% aa identity with mouse, rat and horse TGF-β1. It demonstrates cross-species activity (1). TGF-β1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-β RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-β RI, also known as Activin Receptor-Like Kinase 5(ALK-5), or alternatively, ALK-1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors Betaglycan (also known as TGF-β RIII) and Endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-β in different contexts (11).
|LAP (TGF-beta 1)|
|Flow Cytometry 10 uL/10^6 cells|
|CED, DPD1, LAP, LAP (TGFbeta 1), TGFB, TGFB1, TGFbeta, transforming growth factor beta 1|
|Chinese hamster ovary cell line CHO-derived recombinant mouse TGF-beta 1 Met1-Ser390 Accession # P04202|
|Protein A or G purified from hybridoma culture supernatant|
|Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide|
|Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.|
|Detects mouse LAP (TGF-beta 1) in flow cytometry.|
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