LbCpf1, Mouse anti-Bacteria, Clone: 2D5-6G11, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABE182425UG

Catalog No. MABE182425

Description

Anti-LbCpf1, clone 2D5-6G11 Antibody, Cat. No. MABE1824, is a highly specific mouse monoclonal antibody that targets LbCpf1 and has been tested for use in Western Blotting.

CRISPR-associated endonuclease Cas12a (UniProt: A0A182DWE3; also known as CRISPR-associated endonuclease Cpf1; Lbcpf1) is a single crRNA-guided endonuclease of the class 2 CRISPR-Cas system that lacks tracrRNA and undergoes large conformational changes upon crRNA binding. Cpf1 has several features that can distinguish it from Cas9. Unlike Cas9 that uses dual-guide RNAs, Cpf1 is guided by a crRNA. It recognizes a T-rich PAM (T-rich protospacer adjacent motif), whereas Cas9 favors a G-rich PAM and Cpf1 generates staggered ends at its PAM-distal region, but Cas9 creates blunt ends at its PAM-proximal region. Cpf1 displays higher targeting specificity in mammalian cells when compared to Cas9. It has been shown that depending on the PAM sequences, LbCpf1 can undergo conformational changes to form altered interactions with the PAM-containing DNA duplexes, hence achieving the relaxed PAM recognition. (Ref.: Zetsche, B., et al. (2015). Cell 163(3); 759-77; Yamano, T., et al. (2017). Mol. Cell 67(4); 633-645; Gao, P., et al. (2016). Cell Res. 26(8); 901-913).

Specifications

• Antigen: LbCpf1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Bacteria
• Form: Purified

• Applications: Western Blot
• Clone: 2D5-6G11
• Dilution: Western Blotting Analysis: Various dilutions from a representative lot detected LbCpf1 in lysates from HEK293 cells expressing HA-tagged LbCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
• Gene Alias: Cpf1
• Immunogen: His-tagged recombinant fragment from the N-terminal region of Lachnospiracea bacteria.
• Quantity: 25 μg
• Research Discipline: Epigenetics & Nuclear Function
• Test Specificity: Clone 2D5-6G11 specifically detects CRISPR-associated endonuclease Cas12a (LbCpf1).
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG1 κ