Invitrogen™ LC3A/LC3B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA116931

Catalog No. 01-950-172

Description

This antibody detects both LC3A and LC3B. Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Recommended positive controls: LC3B overexpression lysate, mouse brain whole tissue lysate (adult whole normal), human brain whole tissue lysate (adult whole normal), Neuro2a whole cell lysate, Neuro2a Chloroquine-treated/untreated cell lysate, HeLa chloroquine-treated/untreated cell lysate This antibody is predicted to react with Bovine based on 100% sequence homology.

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

Specifications

• Antigen: LC3A/LC3B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MAP1LC3B2
• Gene Alias: 1010001C15Rik; 1010001H21Rik; 4922501H04Rik; Atg8; ATG8E; ATG8F; Autophagy-related protein LC3 A; autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; autophagy-related ubiquitin-like modifier LC3 B; LC3; LC3a; LC3b; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B light chain 3 B; Map1alc3; MAP1BLC3; Map1lc3; Map1lc3a; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 alpha; microtubule associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3; microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1-light chain 3A; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A; Microtubule-associated proteins 1A/1B light chain 3B; Mpl3; wu:fb60g11; zbs559; zgc:56434; zgc:77094
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100359928, 322425, 362245, 406466, 477201, 479619, 643246, 64862, 66734, 67443, 81631, 84557
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid

• Applications: ChIP Assay, ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: A6NCE7, Q62625, Q6XVN8, Q91VR7, Q9CQV6, Q9GZQ8, Q9H492
• Gene Symbols: MAP1LC3A, MAP1LC3B, MAP1LC3B2
• Immunogen: Synthetic peptide corresponding to a region between residues 25-121 of human LC3 protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Fish, Zebrafish, Plant
• Product Type: Antibody
• Isotype: IgG