LC3C, Rabbit anti-Rat, Polyclonal, Millipore Sigma™

Catalog No.	Quantity
ABC2075200	200 μL
ABC207550UL	50 μL

Catalog No. ABC2075200 Supplier MilliporeSigma Supplier No. ABC2075200UL

Description

Rabbit Polyclonal Antibody

Anti-LC3C, Cat. No. ABC2075, is a rabbit polyclonal antibody that detects Microtubule-associated proteins 1A/1B light chain 3C and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Microtubule-associated proteins 1A/1B light chain 3C (UniProt: Q9BXW4; also known as Autophagy-related protein LC3C, Autophagy-related ubiquitin-like modifier LC3C, MAP1 light chain 3-like protein 3, MAP1A/MAP1B light chain 3 C, MAP1A/MAP1B LC3 C, Microtubule-associated protein 1 light chain 3 gamma) is encoded by the MAP1LC3C gene (Gene ID: 440738) in human. LC3s (MAP1-LC3A, B and C) are structural proteins of autophagosomal membranes, widely used as biomarkers of autophagy. LC3A is mainly localized to perinuclear and nuclear regions, LC3B is evenly distributed throughout the cytoplasm and is also localized in the nucleolar regions, and LC3C is shown to be located in the cytoplasm and strongly in the nuclei. LC3C is synthesized with a propeptide (aa 127-147), which is removed in the mature form. LC3C is a ubiquitin-like modifier that plays a crucial role in antibacterial autophagy through the selective binding of CALCOCO2. It recruits all ATG8 family members to infecting bacteria such as S.Typhimurium. It is also reported to play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins. LC3C is recruited to damaged mitochondria where it mediates autophagosomal degradation of mitochondrial parts in a piecemeal fashion and is required for maintaining basal mitochondrial network homeostasis. It also mediates autophagosomal degradation of MTX1 protein and cooperates with p62 to deliver MTX1 to lysosomes. (Ref.: Koukourakis, KI et al. (2015). PLoS One DOI:10.1371/journal.pone.0137675; Le Guerroue´ et al., 2017, Mol. Cell 68(4); 786-796).

Specifications

• Antigen: LC3C
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected LC3C in rat brain tissue sections.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Microtubule-associated proteins 1A/1B light chain 3C;Autophagy-related protein LC3 C;Autophagy-related ubiquitin-like modifier LC3C;MAP1 light chain 3-like protein 3;MAP1A/MAP1B light chain 3 C;MAP1A/MAP1B LC3 C;Microtubule-associated protein 1 light chain 3 gamma
• Gene Symbols: MAP1LC3C
• Host Species: Rabbit
• Immunogen: GST-tagged full-length human recombinant Microtubule-associated proteins 1A/1B light chain 3C (LC3C).
• Molecular Weight of Antigen: ∽17 kDa observed; 16.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Purification Method: Affinity Purified
• Quantity: 200 μL
• Regulatory Status: RUO
• Research Discipline: Apoptosis & Cancer
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001004343.1
• Test Specificity: This rabbit polyclonal antibody specifically detects LC3C.
• Target Species: Rat
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG