Invitrogen™ LCK Monoclonal Antibody (8E5F9, 4B2C7)

Catalog No.	Quantity
PIMA515295	100 μL

Catalog No. PIMA515295 Supplier Invitrogen™ Supplier No. MA515295

Description

Mouse Monoclonal Antibody

MA5-15295 targets Lck in indirect ELISA, WB applications and shows reactivity with Human samples. The MA5-15295 immunogen is purified recombinant fragment of human Lck expressed in E. Coli. MA5-15295 detects Lck which has a predicted molecular weight of approximately 56kDa.

LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.

Specifications

• Antigen: LCK
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 8E5F9, 4B2C7, 4B2C7
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: ascites with 0.03% sodium azide
• Gene: LCK
• Gene Accession No.: P06239
• Gene Alias: EC 2.7.10.2; Hck-3; IMD22; kinase Lck; Lck; LCK proto-oncogene, Src family tyrosine kinase; Lck1; Lcktkr; leukocyte C-terminal Src kinase; LSK; Lskt; Lsk-t; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte protein tyrosine kinase; lymphocyte-specific protein tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56>lck>; p56lck; p56-LCK; pp58lck; Protein YT16; Proto-oncogene Lck; Proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; t cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; tyrosine-protein kinase Lck; YT16
• Gene Symbols: LCK
• Host Species: Mouse
• Immunogen: Purified recombinant fragment of human Lck expressed in E. Coli.
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3932
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a