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Invitrogen™ Legionella pneumophila subsp. pneumophila (strain Philadelphia 1/ATCC 33152/DSM 7513) sdeA Polyclonal Antibody
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Catalog No. PIPA5117540
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100 μL
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PIPA5117540 100 μL
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Catalog No. PIPA5117540 Supplier Invitrogen™ Supplier No. PA5117540
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Description

Rabbit Polyclonal Antibody

This antibody has been tested in direct-ELISA.

Secreted effector that interferes with the host cell ubiquitin pathway and is required for intracellular bacterial replication. Catalyzes the ubiquitination of several mammalian Rab proteins (Rab33b, Rab1, Rab6a and Rab30) during L.pneumophila infection, without engaging the standard cellular enzyme cascade (E1 and E2). Transfers an ADP-ribose moiety from NAD to the 'Arg-42' residue of ubiquitin in a reaction that releases nicotinamide. The modified ubiquitin is subsequently transferred to the substrate protein through an unknown mechanism that results in the release of AMP. Cannot ubiquitinate the endosomal Rab5 or the cytoskeletal small GTPase Rac1 (PubMed:27049943). Also acts as a deubiquitinase (DUB), catalyzing the cleavage of three of the most abundant polyubiquitin chains ('Lys-11', 'Lys-48' and 'Lys-63') with a distinct preference for 'Lys-63' linkages; is thus able to efficiently remove 'Lys-63'-linked polyubiquitin chains from the phagosomal surface. Is also able to remove NEDD8 from neddylated proteins, but is unable to recognize SUMO. The DUB activity of SdeA is important for regulating the dynamics of ubiquitin association with the bacterial phagosome, but is dispensable for its role in intracellular bacterial replication (PubMed:26598703).
TRUSTED_SUSTAINABILITY

Specifications

Antigen Legionella pneumophila subsp. pneumophila (strain Philadelphia 1/ATCC 33152/DSM 7513) sdeA
Applications Western Blot
Classification Polyclonal
Concentration 2.88 mg/mL
Conjugate Unconjugated
Formulation PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Host Species Rabbit
Immunogen Recombinant Legionella pneumophila subsp Ubiquitinating/deubiquitinating enzyme SdeA protein (19-268aa).
Purification Method Protein G
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Bacteria
Content And Storage -20°C or -80°C if preferred
Product Type Antibody
Form Liquid
Isotype IgG
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Safety and Handling

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