Invitrogen™ Leptin Monoclonal Antibody (11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ ABS0221102

Catalog No. ABA-221102

Description

ABS 022-11-02 detects Leptin in human samples. ABS 022-11-02 has been successfully used in ELISA and Western blot procedures. ABS 022-11-02 binds free Leptin in solution. In sandwich ELISA, ABS 022-11-02 works as a detection antibody with ABS 022-03-02 as a capture antibody. ABS 022-11-02 shows three bands at 16, 32, and 48 kDa in the Western blot application corresponding with the leptin monomer, dimer and trimer forms. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL.

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

Specifications

• Antigen: Leptin
• Classification: Monoclonal
• Concentration: 0.99 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: P41159
• Gene Symbols: Lep
• Immunogen: Purified, recombinant Leptin.
• Quantity: 400 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA
• Clone: 11
• Conjugate: Unconjugated
• Gene: Lep
• Gene Alias: CD295 antigen; H-Leptin; HuB219; Lep; LEPD; LEP-R; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); leptin precursor; Method: conceptual translation supplied by author.; OB; obese; obese protein; obese, mouse, homolog of; obesity; obesity factor; obesity protein; OB-R; OBS; truncated leptin
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 3952
• Content And Storage: 4°C, store in dark
• Form: Liquid