Leptin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P68BT25	Invitrogen™ 500P68BT25UG	25 μg
500P68BT50	Invitrogen™ 500P68BT50UG	50 μg
50-271-8459	Invitrogen™ 500P68BT500UG	500 μg

Description

AA Sequence of recombinant protein: MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine Leptin. Anti-Murine Leptin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Leptin (500-P68) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P68BT-1MG will be provided as 2 x 500 μg

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

Specifications

• Antigen: Leptin
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Mouse
• Gene Accession No.: P41160
• Gene ID (Entrez): 16846
• Immunogen: E.coli-derived Recombinant Murine Leptin.
• Purification Method: Antigen affinity chromatography
• Gene: Lep

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: CD295 antigen; H-Leptin; HuB219; Lep; LEPD; LEP-R; Leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); leptin precursor; Method: conceptual translation supplied by author.; OB; obese; obese protein; obese, mouse, homolog of; obesity; obesity factor; obesity protein; OB-R; OBS; truncated leptin
• Gene Symbols: Lep
• Primary or Secondary: Primary
• Content And Storage: -20°C