Invitrogen™ LGI1 Monoclonal Antibody (N283/7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527652

Catalog No. PIMA527652

Description

1 μg/mL of MA5-27652 was sufficient for detection of LGI1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 60kDa. This antibody was formerly sold as clone S283-7.

The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist.

Specifications

• Antigen: LGI1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.1% sodium azide; pH 7.4
• Gene Accession No.: O95970, Q8K4Y5, Q9JIA1
• Gene Symbols: LGI1
• Immunogen: Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (PFA fixed), Western Blot, Immunocytochemistry
• Clone: N283/7
• Conjugate: Unconjugated
• Gene: LGI1
• Gene Alias: ADLTE; ADPAEF; ADPEAF; BB130740; EPITEMPIN; Epitempin 1; Epitempin-1; EPT; ETL1; IB1099; leucine rich glioma inactivated 1; leucine-rich glioma inactivated 1; leucine-rich glioma-inactivated protein 1; leucine-rich repeat LGI family, member 1; leucine-rich, glioma inactivated 1; LGI1; OTTHUMP00000020121; OTTHUMP00000020122; UNQ775/PRO1569
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 252892, 56839, 9211
• Content And Storage: -20°C
• Form: Liquid