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Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green

Catalog No. r37609
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Quantity:
1 kit

Description

Includes

3 x 2.5mL NucBlue Live, 3 x 2.5mL NucGreen Dead

Determine the viability of cells quickly and easily. Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Green is a ready-to-use assay kit for use in for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.

ReadyProbes™ Cell Viability Imaging Kit, Blue/Green contains NucBlue™ Live reagent (Hoechst 33342) and NucGreen™ Dead reagent. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. NucGreen™ Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC/GFP (green) filter set.

  • NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460nm)
  • NucGreen™ Dead reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC/GFP (green) filter set (excitation/emission maxima: 504/523nm)

Suggestions for use

  • NucBlue™ Live and NucGreen™ Dead reagents may be added directly to cells in full growth media or a compatible buffer solution.
  • In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
TRUSTED_SUSTAINABILITY

Specifications

Cell Type Mammalian Cells, Eukaryotic Cells
Color Blue, Green
Content And Storage Store at 2° to 8°C, or at room temperature.
Description ReadyProbes Cell Viability Imaging Kit, Blue/Green
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope
Product Type Cell Viability Imaging Kit
Dye Type 3 x 2.5 mL NucBlue™ Live
Emission 360/504
Excitation Wavelength Range 460, 523 nm
Format Dropper bottles
Product Line ReadyProbes
Quantity 1 kit
Shipping Condition Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.

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