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Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Red

Catalog No. R37610
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Catalog No. R37610 Supplier Invitrogen™ Supplier No. R37610
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Description

Includes

3 x 2.5mL NucBlue Live, 3 x 2.5mL propidium iodide

Determine the viability of cells quickly and easily. Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Red/Blue is a ready-to-use assay kit for use in for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.

ReadyProbes™ Cell Viability Imaging Kit, Red/Blue contains NucBlue™ Live reagent (Hoechst 33342) and propidium iodide. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected using a standard DAPI filter. Propidium iodide stains only the nuclei of cells with compromised plasma membrane integrity and is detected with a standard TRITC/RFP (orange) filter set.

  • NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460nm)
  • Propidium iodide: stains the nuclei of dead cells with compromised plasma membrane; detected with standard TRITC/RFP (orange) filter set (excitation/emission maxima: 535/617nm)

Suggestions for use

  • NucBlue™ Live reagent and propidium iodide can be added directly to cells in full growth media or a compatible buffer solution.
  • In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
TRUSTED_SUSTAINABILITY

Specifications

Cell Type Mammalian Cells, Eukaryotic Cells
Color Blue, Red
Content And Storage Store at 2° to 8°C, or at room temperature.
Description ReadyProbes Cell Viability Imaging Kit, Blue/Red
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Fluorescence Microscope
Product Type Cell Viability Imaging Kit
Dye Type 3 x 2.5 mL NucBlue™ Live; 3 x 2.5 mL propidium iodide
Emission 460/617
Excitation Wavelength Range 360, 535 nm
Format Dropper bottles
Product Line ReadyProbes
Quantity 1 kit
Shipping Condition Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.

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