LIGHT Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P308BT25	Invitrogen™ 500P308BT25UG	25 μg
50-271-8524	Invitrogen™ 500P308BT500UG	500 μg

Description

AA Sequence of recombinant protein: MRLHQRLGDI VAHLPDGGKG SWEKLIQDQR SHQANPAAHL TGANASLIGI GGPLLWETRL GLAFLRGLTY HDGALVTMEP GYYYVYSKVQ LSGVGCPQGL ANGLPITHGL YKRTSRYPKE LELLVSRRSP CGRANSSRVW WDSSFLGGVV HLEAGEEVVV RVPGNRLVRP RDGTRSYFGA FMV. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine LIGHT. Anti-Murine LIGHT-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Murine LIGHT by sandwich ELISA (using 100 μL/well) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine LIGHT (500-P308) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Murine LIGHT. Western Blot: To detect Murine LIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine LIGHT is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P308BT-1MG will be provided as 2 x 500 μg

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C. elegans death receptor-3) protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.

Specifications

• Antigen: LIGHT
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Mouse
• Gene Accession No.: Q9QYH9
• Gene ID (Entrez): 50930
• Immunogen: E.coli-derived Recombinant Murine LIGHT.
• Purification Method: Antigen affinity chromatography
• Gene: TNFSF14

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: CD258; Herpes virus entry mediator ligand; herpesvirus entry mediator ligand; HVEML; HVEM-L; Light; LTg; Ly113; sCD258; sLIGHT; soluble CD258; soluble LIGHT; Tnfsf14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand 1D; tumor necrosis factor ligand superfamily member 14; Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form; tumor necrosis factor superfamily member 14; UNQ391/PRO726
• Gene Symbols: TNFSF14
• Primary or Secondary: Primary
• Content And Storage: -20°C