Invitrogen™ LIME Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA589384

Catalog No. PIPA589384

Description

Immunogen sequence: MGLPVSWAPP ALWVLGCCAL LLSLWALCTA CRRPEDAVAP RKRARRQRAR LQGSATAAEA SLLRRTHLCS LSKSDTRLHE LHRGPRSSRA LRPASMDLLR PHWLEVSRDI TGPQAAPSAF PHQELPRALP AAAATAGCAG LEATYSNVGL AALPGVSLAA SPVVAEYARV QKRKGTHRSP QEPQQGKTEV TPAAQVDVLY SRVCKPKRRD PGPTTDPLDP KGQGAILALA GDLAYQTLPL RALDVDSGPL ENVYESIREL GDPAGRSSTC GAGTPPASSC PSLGRGWRPL PASLP; Positive Samples: K-562, Mouse lung; Cellular Location: Cell membrane, Single-pass type III membrane protein.

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Specifications

• Antigen: LIME
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Lime1
• Gene Alias: 2810038K19Rik; dJ583P15.4; FLJ20406; Lck interacting transmembrane adaptor 1; lck-interacting membrane protein; Lck-interacting molecule; lck-interacting transmembrane adapter 1; Lime; lime 1; LIME1; LP8067; RP4-583P15.5
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54923, 72699
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.08 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q9EQR5, Q9H400
• Gene Symbols: Lime1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human LIME1 (NP_0602762).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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