Invitrogen™ LIMK2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521987

Catalog No. PIPA521987

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (92%), Rat (92%), Chicken (80%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

LIMK2 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK2 has also been implicated in brain development. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.

Specifications

• Antigen: LIMK2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: LIMK2
• Gene Alias: EC 2.7.11.1; kinase LIMK2; LIM domain kinase 2; LIM kinase 2; LIM motif-containing protein kinase 2; LIMK; LIMK 2; LIMK1; LIMK-1; Limk2; LIMK-2; Limk2b; LINhp-2; Link2; OTTHUMP00000199483
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3985
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P53671
• Gene Symbols: LIMK2
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 252 (P53671) of LIM kinase 2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov