LIN28B Rabbit anti-Human, Polyclonal, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc A303589A

Catalog No. 50-156-5548

Description

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5′-GGAG-3′ motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5′-GGAG-3′ motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Specifications

• Antigen: LIN28B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: LIN28B
• Gene Alias: CSDD2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 389421
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunohistochemistry, Immunoprecipitation
• Concentration: 1 mg/ml
• Formulation: phosphate, tris citrate with 0.09% sodium azide; pH 7-8
• Gene Accession No.: Q6ZN17
• Gene Symbols: LIN28B
• Immunogen: Between 200 and 250
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG