Molecular Probes™ LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation

Description

The LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.

• Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation
• Kit has been optimized and validated for use with a violet laser flow cytometer
• Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time
• Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated
• If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles
• LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs

Low compensation

• The LIVE/DEAD™ Fixable Violet Stain was selected based on its fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser
• The violet-fluorescent reactive dye has an excitation maximum of ∼416 nm and is excited well with the 405 nm violet laser
• It has an emission maxima of ∼451 nm, so it can be collected in the first channel on most violet laser flow cytometers

Specifications

• Cell Permeability: Impermeant
• Cell Type: Eukaryotic Cells
• Color: Violet
• Content And Storage: Contains 2 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO.
  
  Store at -20°C.
  
• Description: LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation
• Detection Method: Fluorescence
• For Use With (Application): Viability Assay
• For Use With (Equipment): Flow Cytometer
• Product Type: Stain
• Dye Type: LIVE/DEAD™ Fixable Violet Dead Cell Stain
• Emission: 451
• Excitation Wavelength Range: 416 nm
• Form: Solid
• Format: Tube(s)
• Product Line: LIVE/DEAD
• Quantity: 80 Assays
• Shipping Condition: Room Temperature
• Solubility: DMSO (Dimethylsulfoxide)

For Research Use Only. Not for use in diagnostic procedures.