Molecular Probes™ LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Catalog No.	Quantity
L34952	200 Assays kit

Catalog No. L34952 Supplier Molecular Probes™ Supplier No. L34952

Description

The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably, and quantitatively distinguish live and dead yeast in minutes by flow cytometry.

The LIVE/DEAD™ FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

Specifications

• Cell Permeability: Impermeant, Permeant
• Color: Green, Red
• Content And Storage: Contains 1 vial of SYTO™ 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO).
  
  Store in freezer (-5°C to -30°C) and protect from light.
  
• Description: LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
• Detection Method: Fluorescence
• For Use With (Application): Viability Assay
• For Use With (Equipment): Flow Cytometer
• Product Type: Yeast Viability Kit
• Dye Type: SYTO™ 9, propidium iodide
• Emission: 498/617
• Excitation Wavelength Range: 485/535 nm
• Form: Solution
• Format: Tube(s)
• Product Line: FungaLight, LIVE/DEAD
• Quantity: 200 Assays kit
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

What are the fluorescence excitation/emission maxima for the SYTO 9 dye and propidium iodide in the LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry?

SYTO 9 dye: 480/500 nm
Propidium iodide: 490/635 nm

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Can I look at bacteria and yeast on a flow cytometer?

Yes, you can. We offer:

-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)

For Research Use Only. Not for use in diagnostic procedures.