Learn More
Molecular Probes™ LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
Description
The LIVE/DEAD™ FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.
Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.
Specifications
Specifications
| Cell Permeability | Impermeant, Permeant |
| Color | Green, Red |
| Content And Storage | Contains 1 vial of SYTO™ 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO). Store in freezer (-5°C to -30°C) and protect from light. |
| Description | LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry |
| Detection Method | Fluorescence |
| For Use With (Application) | Viability Assay |
| For Use With (Equipment) | Flow Cytometer |
| Product Type | Yeast Viability Kit |
| Dye Type | SYTO™ 9, propidium iodide |
| Emission | 498/617 |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.