Invitrogen™ LON Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA580261

Catalog No. PIPA580261

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Escherichia coli LON PROTEASE.

Lon protease is an ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. It is required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Lon protease degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. It binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, UmuD and at least type II antitoxins CcdA, HipB and MazE. Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system. Overproduction leads to very high persister cell formation (cells highly resistant to antibiotics), but not when 10 mRNA interferases are deleted, nor when Lon catalytic residues are mutated.

Specifications

• Antigen: LON
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: ATP-dependent protease La; capR; muc
• Immunogen: Recombinant Escherichia coli LON PROTEASE protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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