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Invitrogen™ LOX-1/OLR Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHOLR1

Encompass

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Catalog No. EHOLR1


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Description

Description

The Human LOX-1 (OLR) ELISA quantitates Hu LOX-1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu LOX-1. Principle of the method The Human LOX-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.
Specifications

Specifications

ELISA Kit
This ELISA pair antibody detects human LOX-1. Other species not determined yet.
Cell Structure & Organelle Proteins
Biotin
P78380
2pg/mL to 500pg/mL
4973
RUO
2°C to 8°C
4 hr. 45 min.
Extracellular Matrix & Cell Adhesion
<12%
96 Tests
Plasma, Serum, Supernatant
CLEC8A,LOX1,LOXIN,SCARE1,SLOX1
Colorimetric Microplate Reader
96 assays
Solid-phase sandwich ELISA
HRP
2.05-500 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
CLEC8A, LOX1, LOXIN, SCARE1, SLOX1
<10%
Human
2 pg/mL
SDS
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