Invitrogen™ LSD1 Monoclonal Antibody (1E5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA524875

Catalog No. PIMA524875

Description

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.

Specifications

• Antigen: LSD1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: KDM1A
• Gene Alias: 1810043O07Rik; AA408884; amine oxidase (flavin containing) domain 2; AOF2; BHC110; BRAF35-HDAC complex protein BHC110; CPRF; D4Ertd478e; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-containing protein 2; KDM1; KDM1A; KIAA0601; Lsd1; lysine (K)-specific demethylase 1; lysine (K)-specific demethylase 1A; lysine demethylase 1A; lysine-specific demethylase 1; lysine-specific histone demethylase 1; lysine-specific histone demethylase 1A; mKIAA0601; neuroprotective protein 3; RGD1562975; RP1-184J9.1; wu:fb82e06; wu:fc51a02; wu:fj36a05; wu:fj66b06
• Host Species: Mouse
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 23028
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 1E5
• Formulation: 0.1M tris glycine with 50% glycerol, 150mM NaCl and 0.2% sodium azide; pH 7.4
• Gene Accession No.: O60341
• Gene Symbols: KDM1A
• Immunogen: Purified recombinant human KDM1A protein fragments expressed in E.coli.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Monkey
• Product Type: Antibody
• Isotype: IgG1