Invitrogen™ LXA4 Competitive ELISA Kit

Description

ELISA

Lipoxin A4 (LXA4) is a potent lipid mediator that plays a crucial role in resolving inflammation and promoting tissue repair. It is a member of the lipoxin family of eicosanoids, which are derived from arachidonic acid metabolism. LXA4 is primarily produced by leukocytes, including neutrophils, monocytes, and macrophages, in response to inflammatory stimuli. LXA4 exerts its anti-inflammatory and pro-resolving effects by binding to specific receptors on target cells, including ALX/FPR2 and GPR32. These receptors are expressed on various immune cells, endothelial cells, and epithelial cells, and their activation leads to the inhibition of pro-inflammatory signaling pathways and the induction of tissue repair processes. The structure of LXA4 is characterized by a 5S, 6R, 15S-trihydroxy-7E, 9E, 11Z, 13E-eicosatetraenoic acid backbone, with a hydroxyl group at position 17 and a carboxylic acid group at position 16. LXA4 is a stereoisomer of another lipoxin, LXB4, which has an opposite configuration at position 5 and a reduced hydroxyl group at position 15.

Specifications

• Assay Range: 0.78 to 50 ng/mL
• Assay Sensitivity: 0.47 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <10%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: LXA4
• Test Time: 1 hrs 20 mins
• Total Assay Time: 3 hrs 30 mins