Invitrogen™ Ly-49A/D Monoclonal Antibody (eBio12A8 (12A8)), PE, eBioscience™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 12578381

Catalog No. 50-108-58

Description

Description: The eBio12A8 antibody reacts with Ly-49A and Ly-49D. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor. eBio12A8 does not react with BALB/c, DBA/2, AKR, or CBA/J Ly-D49D. Applications Reported: This eBio12A8 (12A8) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio12A8 (12A8) antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 5...

The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor.

Specifications

• Antigen: Ly-49A/D
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P20937, Q60651
• Gene Symbols: Klra1, Klra4
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16627, 16635
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBio12A8 (12A8)
• Conjugate: PE
• Gene: Klra1
• Gene Alias: A1; CH29-493D4.3; Chok; class I MHC receptor KLRA22; killer cell lectin-like receptor 4; killer cell lectin-like receptor subfamily A member 32; killer cell lectin-like receptor subfamily A member 33; killer cell lectin-like receptor, subfamily A, member 1; killer cell lectin-like receptor, subfamily A, member 4; Klra1; Klra22; Klra32; Klra33; Klra4; Ly49a; ly-49a; Ly49d; Ly-49d; Ly49-d; Ly49o>129>; ly49r>129>; Ly49v; lymphocyte antigen 49a; lymphocyte antigen 49d; natural killer cell receptor Ly49A; novel killer cell lectin-like receptor subfamily A protein; t lymphocyte antigen A1; T-cell surface glycoprotein Ly-49D; T-cell surface glycoprotein YE1/48
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov