Ly-6A/E Rat anti-Mouse, FITC, Clone: D7, BD

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB562058	BD Biosciences 562058	25 μg
BDB557405	BD Biosciences 557405	0.1 mg

Description

The D7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca-1 (Ly6A/E), a phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cells (HSC) in the bone marrow of mice with both Ly-6 haplotypes. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of lymphocytes from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb (anti-Ly-6A/E, Cat. No. 553333) can block binding of FITC-conjugated D7 antibody (Cat. No. 557405) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat. No. 559971) to identify HSC.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: Ly-6A/E
• Concentration: 0.5mg/mL
• Classification: Monoclonal
• Host Species: Rat
• Target Species: Mouse
• Immunogen: IL-2-dependent mouse T-cell line CTL-L
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: D7
• Applications: Flow Cytometry
• Conjugate: FITC
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG2a κ
• Purification Method: Affinity Purified
• Description: Ly-6A/E; Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Sca-1; TAP

For Research Use Only.