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  6. Ly-6A/E Rat anti-Mouse, FITC, Clone: E13-161.7, BD

Ly-6A/E Rat anti-Mouse, FITC, Clone: E13-161.7, BD

Catalog No. BDB561077 Shop All BD Biosciences Products
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25 μg
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BDB561077 25 μg
BDB553335 0.5 mg
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Description

Rat Monoclonal Antibody

The E13-161.7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca1 (Ly-6A/E), a phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cell (HSC) in mice with both Ly-6 haplotypes. Sca-1+ HSC are found in the adult bone marrow and fetal liver, but not in the early embryo yolk sac or intraembryonic hematopoietic sites, and can be mobilized to the peripheral blood and spleen in the adult. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes, myeloid cells, and thymic and peripheral T lymphocytes, on the earliest intrathymic T-cell precursor population, and in several non-hematopoietic tissues. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of T cells from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody (Cat. No. 557403) have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb can block binding of FITC-conjugated D7 antibody (anti-Ly-6A/E, Cat. No. 557405) to mouse splenocytes, but purified mAb D7 (Cat. No. 557403) is unable to block binding of FITC-conjugated E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat. No. 559971) to identify HSC.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

TRUSTED_SUSTAINABILITY

Specifications

Antigen Ly-6A/E
Applications Flow Cytometry
Classification Monoclonal
Clone E13-161.7
Concentration 0.5mg/mL
Conjugate FITC
Description Sca-1
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Rat
Immunogen BALB/c mouse-derived pre-T cell hybridoma
Purification Method Affinity Purified
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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For Research Use Only.

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