Learn More
Ly-6A/E (Sca-1) Monoclonal Antibody (D7), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
$126.00 - $306.00
Specifications
Antigen | Ly-6A/E (Sca-1) |
---|---|
Clone | D7 |
Concentration | 0.5 mg/mL |
Classification | Monoclonal |
Conjugate | Unconjugated |
Description
Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset. Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and western blotting. Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (Fluorochrome-conjugated D7 is recommended for use in flow cytometry.).
Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.Specifications
Ly-6A/E (Sca-1) | |
0.5 mg/mL | |
Unconjugated | |
Rat | |
Mouse | |
110454, 17069 | |
IgG2a κ | |
Affinity chromatography | |
Antibody |
D7 | |
Monoclonal | |
Liquid | |
RUO | |
P05533, Q64253 | |
Ly6a, Ly6e | |
Primary | |
4°C |
For Research Use Only.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.