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Ly-6A/E (Sca-1) Rat anti-Mouse, Clone: D7, eBioscience™ 
Rat Monoclonal Antibody
$62.00 - $235.00
Specifications
| Antigen | Ly-6A/E (Sca-1) |
|---|---|
| Clone | D7 |
| Target Species | Mouse |
| Conjugate | Unconjugated |
| Isotype | IgG2a, kappa |
Description
Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset. Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and western blotting. Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (Fluorochrome-conjugated D7 is recommended for use in flow cytometry.).
Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Ly-6A/E molecules are implicated in the regulation of T cell activation.Specifications
| Ly-6A/E (Sca-1) | |
| Mouse | |
| IgG2a, kappa | |
| Monoclonal | |
| Liquid | |
| Antibody | |
| 4° C |
| D7 | |
| Unconjugated | |
| Flow Cytometry, Immunoprecipitation, Western Blot | |
| Primary | |
| Monoclonal | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| RUO |
For Research Use Only.
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