Ly-6G/Ly-6C Monoclonal Antibody (NIMP-R14), Invitrogen™

Rat Monoclonal Antibody

Supplier:  Thermo Scientific MA140038

Catalog No. 01-676-494

Description

This clone is highly specific for murine Ly-6G and Ly-6C. It has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with this antibody in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. The endotoxin concentration is < 24 EU/mg, determined with LAL Assay. Suggested positive control: Mouse neutrophils; Suggested negative control: Mouse thymocytes

Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

Specifications

• Antigen: Ly-6G/Ly-6C
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.1% BSA and no preservative
• Gene Accession No.: P0CW02, P0CW03, P35461
• Gene Symbols: Ly6c1, Ly6c2, Ly6g
• Immunogen: Purified BALB/c mouse neutrophils.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: NIMP-R14
• Conjugate: Unconjugated
• Gene: Ly6g
• Gene Alias: AA682074; AA959465; Gr1; Gr-1; Granulocyte Marker; Ly6c; Ly-6C; Ly-6C.2; Ly6c1; Ly-6C1; Ly6c2; Ly-6C2; Ly6g; Ly-6G; ly-6G.1; lymphocyte antigen 6 complex, locus C; lymphocyte antigen 6 complex, locus C1; lymphocyte antigen 6 complex, locus C2; lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6C precursor (Ly-6C); lymphocyte antigen 6C1; Lymphocyte antigen 6C2; lymphocyte antigen 6G; Lymphocyte antigen Ly-6C; lymphocyte differentiation antigen; PML; PMN
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100041546, 17067, 546644
• Content And Storage: 4° C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov