MilliporeSigma™ Ly108/Slamf6, Rat, Unlabeled, Clone: 3E11,

Rat Monoclonal Antibody

Supplier:  MilliporeSigma™ MABF919

Catalog No. MABF919MI

Description

SLAM family member 6 (UniProt Q9ET39; also known as CD352, NK-T-B-antigen, NTB-A, Lymphocyte antigen 108) is encoded by the Slamf6 (also known as Ly108) gene (Gene ID 30925) in murine species. Ly108/Slamf6 belongs to the 7-member (SLAM/CD150, CD48/SLAMF2, Ly-9/CD229, 2B4/CD244, CD84/SLAMF5, Ly108/NTB-A/CD352, CRACC) signaling lymphocytic activation molecule family (SLAMF) of receptors broadly expressed in hematopoietic cells. 2B4 and CD48 interact with eath other, while rest of the family members act as self-ligands, where two identical molecules on the surface of two different cells can interact as receptor and ligand to each other. SLAMF members carry one or more copies of immunoreceptor tyrosine-based switch motif or ITSM (TxYxxV/I) in their cytoplasmic tails. Upon phosphorylation on the tyrosine residue, ITSM mediates the recruitment of SH2 domain-containing signaling molecules such as SLAM-associated protein (SAP), including SAP in T-, NK, and NK T-cells, EAT-2A and EAT-2B (murine) in NK cells and APCs. Ly108/Slamf6 is synthesized with a signal peptide sequence (mouse a.a. 1-30), the removal of which yields the mature Ly108 with an extracellular region (a.a. 31-239) that contains a V-type and a C2-type Ig-like domain (a.a. 36-130 and 147-210), followed by a single transmembrane domain (a.a. 240-262) and a cytoplasmic tail (a.a. 263-351). Ly108/Slamf6 cytoplasmic domain contains two copies of ITSM (mouse a.a. 293-TVYAQV-298 and 317-TIYSIV-322). Alternative splicings produce three Ly108 isoforms, Ly108-1 (Ly108s; UniProt Q9ET39-2), Ly108-2 (Ly108l; UniProt Q9ET39-1) and Ly108-3 (Not reported by UniProt), that differ in their cytoplasmic C-terminal end sequence, but are identical in the rest of their sequence.

Specifications

• Antigen: Ly108/Slamf6
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified rat monoclonal IgG2a in buffer containing PBS without preservatives.
• Gene Accession No.: Q9ET39
• Immunogen: Murine Ly108-human IgG1 Fc fusion protein.
• Quantity: 100 μg
• Research Discipline: Inflammation & Immunology
• Gene ID (Entrez): NP_109635.1
• Target Species: Mouse
• Form: Purified

• Applications: Functional Assay, Immunoprecipitation, Western Blot
• Clone: 3E11
• Conjugate: Unconjugated
• Gene: Slamf6, Ly108
• Host Species: Rat
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 3E11 specifically detected exogenously expressed murine Ly108-1 (Ly108s; UniProt Q9ET39-2) and Ly108-2 (Ly108l; UniProt Q9ET39-1), but not other SLAM family members (2B4, CD84, CRACC, Ly-9, and SLAM) on the surface of respective mouse BI-141 T cell transfectants. Murine Ly108 spliced isoforms Ly108-1 (Ly108s; UniProt Q9ET39-2), Ly108-2 (Ly108l; UniProt Q9ET39-1) and Ly108-3 (Not reported by UniProt) differ in their cytoplasmic C-terminal end sequence, but are identical in the rest of their sequence, including the extracellular region targeted by clone 3E11 (Zhong, M.C., and Veillette, A. (2008). J. Biol. Chem. 283(28):19255 -19264).
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.