Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3), Alexa Fluor™ 647, Novus Biologicals™

Rabbit Monoclonal Antibody

Supplier:  Novus Biologicals NBP281105AF647

Catalog No. NB016190

Description

Lymphotoxin-alpha/TNF-beta Monoclonal specifically detects Lymphotoxin-alpha/TNF-beta in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA.

Specifications

• Antigen: Lymphotoxin-alpha/TNF-beta
• Classification: Monoclonal
• Conjugate: Alexa Fluor 647
• Formulation: 50mM Sodium Borate
• Gene Symbols: LTA
• Immunogen: 3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTalpha expressed in E. coli - the hybridoma clone was selected for its ability to bind LTalpha3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
• Quantity: 0.1 mL
• Research Discipline: Adaptive Immunity, Apoptosis, Asthma, Biologically Active Proteins, Cytokine Research, Immunology, Innate Immunity
• Gene ID (Entrez): 4049
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA
• Clone: 3F12.2D3
• Dilution: Western Blot, Flow Cytometry, ELISA
• Gene Alias: LT, LT-alpha, lymphotoxin alpha (TNF superfamily, member 1), TNFBlymphotoxin-alpha, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1
• Host Species: Rabbit
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: 3F12 binds to human LTalpha3 and LT-alpha-beta (with a KD of ∽0.3 nM, determined by BIACORE; ∽ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTalpha belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTalpha3), or is expressed on the cell surface in complex with LTbeta. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTalpha3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner.
• Content And Storage: Store at 4°C in the dark.
• Isotype: IgG κ