MilliporeSigma™ LysargiNase

Description

MilliporeSigma™ LysargiNase is suitable for peptide or protein digestion in solution or in gel.

• LysargiNase is a metalloproteinase found in the thermophilic archaea Methanosarcina acetivorans
• It specifically cleaves before lysine and arginine residues in proteins
• This cleavage results in peptides with molecular weights similar to tryptic peptides, but with N-terminal lysine or arginine residues that can be fragmented with b ion-dominated spectra
• This can improve protein C-terminal peptide identification and several arginine rich phosphosite assignments
• Unlike with trypsin, LysargiNase cleavage also occurs at methylated lysine and arginine, allowing detection of these epigenetic modifications
• For peptide or protein digestion, a ratio of between 1:100 to 1:20 (w/w) enzyme:substrate is recommended
• It is active up to 55°C at pH 6 to 9
• LysargiNase is reversibly inhibited by 1, 10-phenanthroline, EDTA and other calcium and zinc chelating agents
• It is active under a variety of solvent conditions including 5 mM TCEP, 5% methanol, 5% acetonitrile, 0.8 M urea, 0.1% RapiGest, 1% deoxycholate, 0.2% SDS and 1% NP-40

Specifications

• Description: Mirrors trypsin for protein C-terminal and methylation-site identification
• Quantity: 1/Ea.
• Kit Contents: 20 μg LysargiNase, prepared by recombinant expression in E. coli and lyophilized from buffer
• For Use With (Application): Suitable for peptide or protein digestion in solution or in gel
• Content And Storage: Store the lyophilized product at -20°C for up to 2 years. After reconstitution, the unused material can be stored in the freezer in aliquots. Avoid repeated freeze thaw cycles.