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Invitrogen™ LysoLight™ Antibody Labeling Kits
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Catalog No. L36001
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Quantity:
3 x 1 mg
3 x 100 μg
500 μg
Product Type:
Amine Reactive Dye
Antibody Labeling Kit
Color:
Deep Red
Green

The LysoLight Antibody Labeling Kits offer an efficient method for conjugating antibodies with LysoLight dyes. This process allows for specific and sensitive detection of internalized antibodies within lysosomes using fluorescence microscopy, flow cytometry, or high content imaging platforms.

LysoLight Antibody Labeling Kits provide a powerful tool for visualizing the lysosomal localization and catabolic degradation of antibodies and proteins. These kits utilize a unique fluorophore that is only active upon enzymatic cleavage by the lysosomal specific enzyme, Cathepsin B. Outside of the lysosome, LysoLight dyes have little to no observable background fluorescence, and only upon localization to the protease rich environment of the lysosome, does LysoLight become activated and brightly fluoresce bright green or deep red in color.

The labeling protocol provided in the LysoLight Antibody Labeling Kits involves a simple and rapid procedure, ensuring minimal hands-on time and reliable results. The kits include all necessary reagents, including the LysoLight dye, buffers, and purification columns, to facilitate the antibody labeling process. The LysoLight reactive dye contained in these kits readily reacts with lysine residues in antibodies or proteins to yield a covalently attached fluorogenic sensor. The spin columns included in the kits are used for purifying the labeled antibody from excess dye with yields of 70–95%. The kits contain sufficient reagents for three labeling reactions of 100 μg or 1 mg of antibody. The user guide describes a general protocol for using the amine-reactive form of the LysoLight dye for labeling either 100 μg or 1 mg of IgG.
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Specifications

Color Deep Red
Description LysoLight™ Deep Red Antibody Labeling Kit
Detection Method Fluorescence
For Use With (Application) Antibody and Protein Labeling
For Use With (Equipment) Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument
Product Type Antibody Labeling Kit
Green Features Enhanced stability
Product Line LysoLight
Quantity 3 x 100 μg
Solubility DMSO (Dimethylsulfoxide)
Excitation/Emission 650/668 nm
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For Research Use Only.

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