M-CSF Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA595732

Catalog No. PIPA595732

Description

Immunogen sequence: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQD VVTKPDCN; Positive Samples: BxPC-3, Jurkat, K-562; Cellular Location: Cell membrane, Single-pass type I membrane protein, extracellular space

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

Specifications

• Antigen: M-CSF
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Csf1
• Gene Alias: C87615, colony stimulating factor 1, colony stimulating factor 1 (macrophage), colony-stimulating factor-1 splice variant, CSF1, CSF-1, Csfm, H-MCSF, lanimostim, macrophage colony stimulating factor 1, macrophage colony stimulating factor alpha, macrophage colony stimulating factor beta, macrophage colony-stimulating factor, macrophage colony-stimulating factor 1, macrophage colony-stimulating factor beta, macrophage-colony stimulating factor alpha, MCSF, MCSF alpha, MCSFBETA, MGC31930, M-MCSF, op, osteopetrosis, Processed macrophage colony-stimulating factor 1
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12977, 1435, 78965
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.78 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal, pH 7.3
• Gene Accession No.: P07141, P09603, Q8JZQ0
• Gene Symbols: Csf1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-190 of human CSF1 (NP_7573511)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG